Processes of Recombinant DNA Technology (NEET aspect) скачать в хорошем качестве

Processes of Recombinant DNA Technology (NEET aspect)

Recombinant DNA technology involves altering genetic material by introducing a foreign DNA into a host organism. 1. Isolation of Genetic Material (DNA) DNA is isolated from: Donor organism (gene of interest) Host organism (usually plasmid DNA) DNA is released by breaking the cell using: Enzymes like lysozyme (bacteria) Cellulase (plants) DNA is purified by removing proteins and RNA. 2. Cutting of DNA at Specific Locations Done using restriction endonucleases These enzymes cut DNA at specific palindromic sequences Produces: Sticky ends or blunt ends Same enzyme cuts both: Foreign DNA Vector DNA ensures proper joining Example: EcoRI 3. Amplification of Gene of Interest (PCR) Gene is multiplied using Polymerase Chain Reaction (PCR) Requires: Template DNA Primers Taq polymerase dNTPs Steps: Denaturation Annealing Extension 4. Ligation of DNA Fragment The foreign DNA is joined to the vector using DNA ligase Forms recombinant DNA Sticky ends help in easy ligation 5. Insertion of Recombinant DNA into Host Cell (Transformation) Recombinant DNA is introduced into host (commonly E. coli) Methods: Heat shock Electroporation Microinjection Host cell takes up recombinant DNA 6. Selection and Screening of Recombinants Only cells with recombinant DNA are selected Methods include: Antibiotic resistance markers Blue-white screening Non-recombinants are eliminated 7. Obtaining the Foreign Gene Product Host cells are cultured on a large scale Gene expresses desired protein Product is extracted and purified 8. Downstream Processing Separation and purification of product Formulation into: Medicines Vaccines Enzymes Subscribe 'Crack NEET Biology' to learn the Biology concepts easily with focus on NEET. Thanks for watching my lecture ‎⁨@crackNEETBiologyy⁩ #crackNEETBiology #cbse #Ncert #11th Biology #12th Biology #neetrepeaters #NEET2025 #neetpyqbiology #neetpyqseries #NEET2026 The video-lectures are delivered to help the 11th and 12th students understand the Biology concepts in simple and effective manner, also focused towards previous year NEET questions, enabling students to successfully attempt NEET Biology examination. The lectures are arranged chapter wise for 11th and 12th NCERT Biology in the playlist. You may kindly comment in the topics where you have doubts or need a detailed explanation. The lectures are delivered by S.Pratheeba, M.Tech in Biopharmaceutical Technology, excelled with distinction from AC Tech, Anna University, Chennai. #Processes_Of_Recombinant_DNA_Technology #Biotechnology:PrinciplesandProcesses #PolymeraseChainReaction #PCR #Bioreactors #RecombinantProteins #StirredTankBioreactor #DownstreamProcessing #Biotechnology #GeneticEngineering #GelElectrophoresis #Agarose_Gel_Electrophoresis #TOOLS_OF_RECOMBINANT_DNA_TECHNOLOGY #ProcessesOfRecombinantDNATechnology #Processes_Of_Recombinant_DNA_Technology #neet2026 #Ncertbiology #NCERTbiology #RestrictionEnzymes #RestrictionEndonucleases #Recombinant_DNA_Technology #CloningVectors #CloningSites #SelectableMarkers #TiPlasmid #Retroviruses #CompetentHost #NEETbiologytamil #neetbiologytamil #NEET2026 #NEETpyqseries #NEETpreparation #Introduction #BasicConcept #NEETpreparation #NEET2026 ##NEETpyqseries #NEET2027 #NEETrepeaters #Biotechnology:PrinciplesandProcessesClass12 #Class12 #BIOTECHNOLOGY:PRINCIPLESANDPROCESSES