AIRR C Seminar Series, Jan 30, 2025 Mikhail Pogorelyy, St Jude Children's Research Hospital, USA скачать в хорошем качестве

AIRR C Seminar Series, Jan 30, 2025 Mikhail Pogorelyy, St Jude Children's Research Hospital, USA

Talk title: TIRTL-seq: Deep, quantitative, and affordable paired TCR repertoire sequencing Established Career Speaker: Mikhail Pogorelyy, St. Jude Children's Research Hospital Talk Abstract TCRs are generated by random recombination to recognize a wide variety of pathogens. The distribution of T-cell clones follows a power law, with few large clones and many small ones. Traditionally, TCR repertoire sequencing is costly and limited, as it does not always allow to know the paired alpha and beta chain. TIRTL-seq is a TCR sequencing method that combines high depth, accuracy and low cost. It uses a combinatorial strategy to match alpha and beta chains without the need for single cell sequencing. This allows to study immune responses in more detail and on a large scale. Speaker Bio Mikhail V. Pogorelyy is Director of Computational Science at St. Jude Children's Research Hospital, specializing in adaptive immunity and TCR repertoire analysis. His research develops experimental and computational approaches for deep sequencing of TCRs and BCRs, applied to immunotherapy, gene therapy and infectious diseases. During his postdoc at St. Jude, he created a method to identify TCRs that recognize novel epitopes. He obtained his PhD from the Russian Academy of Sciences.