Ali Shaib | Visualizing Protein Shapes by Expansion Microscopy скачать в хорошем качестве

Ali Shaib | Visualizing Protein Shapes by Expansion Microscopy

Apply to join Foresight Molecular Machines program: https://foresight.org/molecular-machi... A group of scientists, entrepreneurs, and institutional allies who cooperate to advance molecular machines, applications in energy, medicine, and material science, and long-term progress toward Richard Feynman’s vision of nanotechnology. Ali Shaib | Visualizing Protein Shapes by Expansion Microscopy Bio: Dr. Ali Shaib, a Group Leader at the Institute of Neurophysiology, University Medical Center Göttingen, his current research focus on the field of neurological diagnostics. He developed a new affordable super-resolution imaging concept which he will introduce for us today. Beyond microscopy, His research group investigates the world of liquid-liquid phase separation mechanisms and unravels their protein 3D structures at an Ångström-resolution. Leveraging state-of-the-art artificial intelligence and computer vision, they analyze complex data to advance the understanding of neurological diseases, namely Parkinson's disease. Abstract: Fluorescence imaging is one of the most versatile and widely-used tools in biology1. Although techniques to overcome the diffraction barrier were introduced more than two decades ago, and the nominal attainable resolution kept improving2, 3, fluorescence microscopy still fails to image the morphology of single proteins or small molecular complexes, either purified or in a cellular context4, 5. Here we report a solution to this problem, in the form of one-step nanoscale expansion (ONE) microscopy. We combined the 10-fold axial expansion of the specimen (1000-fold by volume) with a fluorescence fluctuation analysis6, 7 to enable the description of cultured cells, tissues, viral particles, molecular complexes and single proteins. At the cellular level, using immunostaining, our technology revealed detailed nanoscale arrangements of synaptic proteins, including a quasi-regular organisation of PSD95 clusters. At the single molecule level, upon main chain fluorescent labelling, we could visualise the shape of individual membrane and soluble proteins. Moreover, conformational changes undergone by the ∼17 kDa protein calmodulin upon Ca2+ binding were readily observable. We also imaged and classified molecular aggregates in cerebrospinal fluid samples from Parkinson’s Disease (PD) patients, which represents a promising new development towards improved PD diagnosis. ONE microscopy is compatible with conventional microscopes and can be performed with the software we provide here as a free, open-source package. This technology bridges the gap between high-resolution structural biology techniques and light microscopy, and provides a new avenue for discoveries in biology and medicine. https://x.com/AliHShaib https://www.rizzoli-lab.de/one-micros... Timecodes 00:00 Introduction and Welcome 01:19 The Evolution of Super-Resolution Microscopy 03:41 Introduction to One Microscopy 04:24 Expansion Microscopy Techniques 13:34 Applications and Examples 16:44 Comparisons with Other Techniques 27:14 Artificial Intelligence in Microscopy 32:27 Reproducibility and Collaborations 35:55 Conclusion and Q&A ══════════════════════════════════════ About The Foresight Institute The Foresight Institute is a research organization and non-profit that supports the beneficial development of high-impact technologies. Since our founding in 1986 on a vision of guiding powerful technologies, we have continued to evolve into a many-armed organization that focuses on several fields of science and technology that are too ambitious for legacy institutions to support. From molecular nanotechnology, to brain-computer interfaces, space exploration, cryptocommerce, and AI, Foresight gathers leading minds to advance research and accelerate progress toward flourishing futures. We are entirely funded by your donations. If you enjoy what we do please consider donating through our donation page: https://foresight.org/donate/ Visit https://foresight.org, subscribe to our channel for more videos or join us here: • Twitter:   / foresightinst   • Facebook:   / foresightinst   • LinkedIn:   / foresight-institute