Massive expansion and biobanking of stem cell derived cardiomyocytes | UMC Utrecht скачать в хорошем качестве

Massive expansion and biobanking of stem cell derived cardiomyocytes | UMC Utrecht

Stem cell-derived cardiomyocytes can be utilized in various strategies; such as cell injections, 3D cellular patches or scaffolds, or paracrine stimulation. A major limitation in the field, however, is the line-to-line variability in the efficiency of stem cell-derived cardiomyocytes generation and the inability to effectively expand cardiomyocytes numbers. In this video, we described a cost-effective strategy for the subsequent massive expansion (of over 250 fold) from high-purity cardiomyocytes. This effect is relying on two main aspects, the removal of cell-cell contacts and WNT activation. This protocol maintains cardiac functionality, allows cryopreservation, and the cells can be used in downstream assays such as disease modelling, drug and toxicity screening, and cell therapy. Read the paper here: https://www.sciencedirect.com/science...