Incucyte® Tutorial Series: Learn How to Remove Background Fluorescence When Image Processing скачать в хорошем качестве

Incucyte® Tutorial Series: Learn How to Remove Background Fluorescence When Image Processing

Incucyte® Live Cell Analysis Tips and Tricks: Background florescence can occur due to the cell culture media components or other artifacts in media. Incucyte® software offers integrated image processing techniques—such as Top-Hat and Surface Fit Background Subtraction—to correct for background fluorescence. This video explores: Top-Hat Background Subtraction Surface Fit Background Subtraction How to Use Autoscale Key Moments: 0:38 Top Hat Background Subtraction: Top Hat Background Subtraction can be explained by the moving disk concept to determine genuine signal the user defines a disk based on the radius of the largest fluorescent object within an image.    1:43 Surface Fit Background Subtraction: The option to choose the surface fit background subtraction and segmentation algorithm. The user will designate a threshold intensity value without defining object size.    2:15 Auto scale: Autoscale is a fluorescence visualisation tool that chooses the minimum and maximum intensity display settings based on the range of pixel intensities in the images. Vessel Autoscale applies a single scale range to each image based on the minimum and maximum brightness across the entire vessel. Follow us on LinkedIn:   / incucyte-live-cell-analysis-systems   Be sure to subscribe to have better access to our videos! #incucyte #livecellanalysis #livecellimaging