Скачать с ютуб Thou Shall: Reference Control Commandments for Cytometry - By Oliver Eltherington в хорошем качестве

Thou Shall: Reference Control Commandments for Cytometry - By Oliver Eltherington

0:00 Introduction to Spectral Cytometry 2:21 Rules for good fluorochrome reference controls 3:18 Rule #1 Reference spectral purity and identity 4:01 R1----Using fluorochrome references as surrogate for sample fluorochromes 10:07 R1----Non-tandem vs. tandem fluorochromes 15:36 R1----Minor differences in instrumentation 18:52 R1----Beads vs. cell-based references 20:00 R1----Summary and further examples of rule breakers 21:19 Rule #2 Reference brightness 21:55 R2----Why reference brightness matters? 25:06 R2----Gating on relevant bright events 26:13 R2----Visualizing rare bright events on histograms 26:53 R2---Importance of parameter scaling on rare event visualization 28:50 Rule #2 summary 29:20 Rule #3 The autofluorescence of fluorescence negative and positive events must be identical 30:00 R3----Autofluorescence mismatch leads to unmixing issues 35:26 R3----Reusing negative controls 37:45 R3----References based on pan cell markers 39:21 Further resources