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Creating Bacterial Glycerol Stocks

Racheal, a Lab Tech here at Addgene, shows you how to create a glycerol stock solution to store your plasmids indefinitely. For the full protocol text, visit http://www.addgene.org/protocols/crea... Bacterial glycerol stocks are important for long-term storage of plasmids. The addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria can be stored stably at -80°C for many years. This protocol begins after you’ve already created a bacterial culture, so please see out video on Inoculating Liquid Bacterial Culture. http://www.addgene.org/protocols/inoc... ___________________________________________________________________________ 0:00 Intro & Overview 1:22 Materials 2:27 Creating the Bacterial Glycerol Stocks 3:39 Recovering Bacteria from your Glycerol Stocks ___________________________________________________________________________ Featuring: Racheal Komuhendo Written, Directed, & Edited by: Quintin Marcelino Music by: Lauren Duski, Vibe Mountain, and RKVC