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The Nextflow Podcast is back! After a brief hiatus, Phil Ewels returns with a comprehensive look at everything that happened in 2025 across the Nextflow ecosystem. Joining Phil for this year-in-review are Rob Syme (Scientific Support Lead), Marcel Ribeiro Dantas (Senior Developer Advocate), and Rob Newman (Product Manager Lead) from Seqera. In this episode, we cover: Nextflow Language Evolution - The new syntax parser, VSCode extension with native language server support, nextflow lint and format commands, and the game-changing Lineage tracking feature for full data provenance. Plugin Ecosystem - The new plugin registry at registry.nextflow.io, easier plugin development, and the complete AWS SDK v2 rewrite for dramatically improved S3 performance at scale. Workflow Inputs and Outputs - How workflow outputs are replacing publishDir, typed parameters, and the foundations being laid for static types in Nextflow. Seqera Platform Updates - The redesigned pipeline run details page, single-VM compute environments with 4-6x faster startup, Seqera Compute, Fusion Snapshots for spot instance resilience, Studios improvements, and Data Explorer S3 API support. AI and Automation - Seqera AI developments, the MCP server for integrating with Claude/Cursor/Copilot, and Node-RED integration for pipeline chaining and automation. Community Growth - nf-core tools releases, the syntax adoption roadmap, Nextflow Summits in Boston and Barcelona, the Ambassador program expanding to 40 countries, Training Weeks, and Seqera's Series B funding. Links mentioned in this episode: Nextflow Plugin Registry: https://registry.nextflow.io Node-RED Integration: https://seqera.io/blog/node-red/ Seqera MCP Server: https://seqera.io/blog/seqera-mcp/ nf-core Syntax Roadmap: https://nf-co.re/blog/2025/nextflow_s... Fusion Snapshots: https://seqera.io/blog/fusion-snapsho... Seqera Compute: https://seqera.io/blog/seqera-compute... Subscribe to stay updated on all things Nextflow, and check out the full show notes at seqera.io/podcast 00:00 Nextflow Podcast - Episode 53: 2025 Recap 00:09 Welcome 01:10 Introductions 02:54 Nextflow Language Evolution 03:44 Nextflow VSCode Extension 06:47 Nextflow lint and format 08:21 Nextflow Lineage 12:33 Nextflow Plugin Registry 17:29 AWS SDK v2 upgrade 21:09 Workflow Inputs/Outputs and Static Types 26:38 Seqera Platform: Pipeline Run Details 29:57 Dynamic resource labels 30:56 Pipeline versioning 31:58 Pipeline Chaining with Node-RED 35:07 Single VM Compute Environments 39:14 Seqera Compute 41:24 Fusion Snapshots 44:32 Seqera AI 49:03 Seqera MCP Server 50:04 Data Explorer & s3 APIs 51:24 Datasets 52:54 Seqera Studios Updates 56:56 Seqera company updates 57:31 nf-core/tools updates 58:41 Topics 01:00:05 Syntax updates in nf-core 01:00:55 Community growth 01:03:43 Nextflow Summits and events 01:05:00 Nextflow Ambassadors 01:07:19 Training Weeks 01:09:13 Wrap up