Experimental methods for identifying synergistic nanobody combinations that neutralize SARS-CoV-2 скачать в хорошем качестве

Experimental methods for identifying synergistic nanobody combinations that neutralize SARS-CoV-2

Abstract of this webinar： The COVID-19 pandemic has underscored the need for superior, highly effective and variant-resistant therapeutics against emerging viral pathogens. Nanobodies, recombinantly expressed fragments of antibodies produced by llamas and other members of the camelid family, are an exciting candidate for therapeutic development. They are ten times smaller than human antibodies, remarkably robust, and can be readily “humanized” for use in diagnostic and therapeutic treatments. Nanobodies can bind to viral antigens in places not accessible to human antibodies and are considerably more thermo-resistant. We have generated a repertoire containing greater than100 nanobodies against the spike protein of SARS-CoV-2. From this repertoire, we have identified distinct nanobody classes that have exceptional biophysical characteristics and unique and orthogonal mechanisms for neutralizing SARS-CoV-2. In this webinar, we will discuss the advantages of nanobodies and our methods to characterize their neutralizing activity, including use of liquid-handling robots and methods to readout neutralization efficacy. Using these methods we have identified several nanobody combinations that display drug-like synergy, enhancing neutralizing activity far greater than expected by additive activity alone. These results offer insights into optimal design principles for developing highly effective and variant-resistant COVID-19 therapeutics. The speakers will discuss: a) Nanobodies are effective variant-resistant neutralizers of SARS-CoV-2 b) Combinations of nanobodies with orthogonal mechanisms of neutralization are highly synergistic in neutralization activity c) Automation of liquid handling improves assay development and experimental results About Bio-protocol Webinars： The Bio-protocol webinar series targets biomedical and life science developments and advances, focusing on a detailed presentation of cutting-edge technologies and techniques. We aim to make this program a go-to place for sharing, learning, discussing, and uncovering how to apply new technologies and tools in the life science community. Visit the Bio-protocol website to learn more about our webinar series. Subscribe to our channel:    / @bio-protocolwebinars