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Watch the Full Video at https://www.jove.com/v/54078/modeling.... Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells - a 2 minute Preview of the Experimental Protocol Rui Liang, Wei Dong, Xiaopeng Shen, Xiaoping Peng, Angie G. Aceves, Yu Liu University of Houston, Department of Biology and Biochemistry; University of Houston, Department of Economics; The First Affiliated Hospital of Nanchang University, Department of Cardiology; In this protocol, we present the procedures in establishing myotonic dystrophy 1 myoblast models, including optimized C2C12 cell maintenance, gene transfection/transduction, and myocyte differentiation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments