Don't let your electrophoresis gels smile - gel electrophoresis voltage, heat, buffers etc. скачать в хорошем качестве

Don't let your electrophoresis gels smile - gel electrophoresis voltage, heat, buffers etc.

Gel smiling is often caused by uneven heating of your gel, often because you’re running at too high of voltage. Tips to avoid it: run at a lower voltage use a buffer with lower electrolyte concentrations (e.g. 0.5X TBE instead of 1X or one of the newer Tris-less buffers like sodium borate (SB)) load 1X buffer in your empty lanes pre-run gels use a metal plate to even out heat distribution run in cold room or use ice pack in gel box make sure your buffer is well-mixed make sure your inner buffer chamber isn’t leaking wash out gel wells (especially for urea-PAGE) I don’t have time to write text, but here are some resources: History and principles of conductive media for standard DNA electrophoresis (2004) Jonathan R. Brody & Scott E. Kern, Analytical Biochemistry https://doi.org/10.1016/j.ab.2004.05.054 Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis (2018) Jonathan R. Brody & Scott E. Kern, BioTechniques https://doi.org/10.2144/04362BM02 Top 10 Fun Facts for DNA Electrophoresis, Reproduced from Life Technologies, Inc., Shanta Dube: https://bio.davidson.edu/courses/Molb... Constant Current or Voltage in SDS-PAGE: The Great Debate, Zandile Nare, BiteSize Bio, April 15, 2021 https://bitesizebio.com/51744/constan... Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis.  (2014) Sanderson, B. A., Araki, N., Lilley, J. L., Guerrero, G., & Lewis, L. K. Analytical biochemistry, 454, 44–52. https://doi.org/10.1016/j.ab.2014.03.003 more on nucleic acid PAGE: blog form: http://bit.ly/ureapage ; YouTube:    • Nucleic acid PAGE (Polyacrylamide Gel Elec...   sd more on agarose gel electrophoresis: http://bit.ly/agarosegelrunning more on SDS-PAGE: http://bit.ly/sdspageruler                       more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com