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Watch the Full Video at https://www.jove.com/v/55659/determin.... Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms - a 2 minute Preview of the Experimental Protocol Danlin Yang, Ajit Singh, Helen Wu, Rachel Kroe-Barrett Boehringer Ingelheim Pharmaceuticals, Inc., Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery; Columbia University, The Fu Foundation School of Engineering and Applied Science; We describe here protocols for the measurement of antibody-antigen binding affinity and kinetics using four commonly used biosensor platforms. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments