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What cofactors are needed to synthesize and recycle BH4?

Chris Masterjohn, PhD is the Founder and Scientific Director of the mitochondrial test mito.me. Question: What cofactors are needed to synthesize and recycle BH4? Short Answer: Zinc, magnesium, potassium, and niacin are the cofactors needed for the synthesis and recycling of BH4. Folate and methylation are not involved, though high-dose folate or folic acid could hypothetically hurt BH4 recycling since both are recycled by dihydrofolate reductase (DHFR). This is a clip from a live Q&A session open to CMJ Masterpass members. In addition to this episode, you can access two other free samples using this link: https://chrismasterjohnphd.substack.c... In that batch of free episodes you will also find the answers to these questions: *Is It Important to Get Vitamin D Sulfate Specifically From the Sun? *How Much Iron Can We Absorb At Once? If you want to become a Masterpass member so you can participate in the next live Q&A, or so you can have access to the complete recording and transcript of each Q&A session, you can save 10% off the subscription price for as long as you remain a member by using this link to sign up: https://chrismasterjohnphd.substack.c... Learn more about the Masterpass here: https://chrismasterjohnphd.substack.c... This snippet is from the February 15, 2023 AMA. The full recording and transcript is reserved for Masterpass members. Here is a preview of what’s included: *Is It Important to Get Vitamin D Sulfate Specifically From the Sun? *How Much Iron Can We Absorb At Once? *What nutrients are important for long-term PPI use? *For how long does transferrin saturation respond to recent iron-rich food? *Muscle spasms: creatine, creatinine, sodium, and potassium. *Hematologists ignore iron saturation. *How to detox arsenic? *Could folic acid supplements impair BH4 recycling? *How to increase butyrate? *More on hematologists and transferrin saturation. Here’s a link to the full AMA: https://chrismasterjohnphd.substack.c... For more information, visit https://www.chrismasterjohn-phd.com/ Do You Have Hidden Mitochondrial Dysfunction? Mitochondrial dysfunction is one of the earliest signs of aging and chronic disease—and most people don’t know they have it. Mitome is the first at-home test that measures your cellular energy directly and gives you a personalized roadmap to optimize energy, slow aging, and protect against disease. Find my comprehensive mitochondrial analysis at mito.me