26N. Invited Speaker - Jonas Ries скачать в хорошем качестве

26N. Invited Speaker - Jonas Ries

Quantitative high-throughput single-molecule localization microscopy 26th of November, 2020. by Jonas Ries, Group Leader at EMBL-Heidelberg (DE). Chairs: Gaby Martins & Pedro Pereira Abstract: Single-molecule localization microscopy (SMLM) is becoming increasingly widespread in biological labs. While it holds enormous potential for biological discovery, it is a complex imaging technique unforgiving to imperfect image acquisi¬tion, sample quality, and computational analyses. Here, I will introduce reference standards based on the nuclear pore complex for quality that allow quantifying resolution and performance of the microscope, optimization of the entire imaging workflow including the labeling efficiency and counting of proteins in an unknown complex. In a second part, I will report on our efforts to increase throughput and speed in SMLM.