Micro CT Imaging of the Tobacco Hornworm Caterpillar : General Anatomy of the Alimentary Tract скачать в хорошем качестве

Micro CT Imaging of the Tobacco Hornworm Caterpillar : General Anatomy of the Alimentary Tract

Volume rendering of an early L5d2 Manduca sexta larva with an introduction to the general anatomy of the alimentary tract.    • A quantitative micro-tomographic gut atlas...   For more information, check out our paper: A Quantitative Micro-Tomographic Gut Atlas of the Lepidopteran Model Insect Manduca sexta Anton G. Windfelder, Jessica Steinbart, Ulrich Flögel, Marian Kampschulte, Gabriele A. Krombach and Andreas Vilcinskas Open AccessPublished:May 04, 2023 https://doi.org/10.1016/j.isci.2023.1... Highlights: • This is a quantitative 3D reference atlas of the digestive tract of M. sexta • Oral application of iodixanol allowed a high-resolution 3D analysis of the gut • Revealing structures like the crop, gastric ceca or the complex gut folding pattern • Gut volumes can be used to calculate local concentrations of oral-applied compounds Summary: The tobacco hornworm is used extensively as a model system for ecotoxicology, immunology and gut physiology. Here, we established a micro-computed tomography approach based on the oral application of the clinical contrast agent iodixanol, allowing for a high-resolution quantitative analysis of the M. sexta gut. This technique permitted the identification of previously unknown and understudied structures, such as the crop or gastric ceca, and revealed the underlying complexity of the hindgut folding pattern, which is involved in fecal pellet formation. The acquired data enabled the volume rendering of all gut parts, the reliable calculation of their volumes, and the virtual endoscopy of the entire alimentary tract. It can provide information for accurate orientation in histology uses, enable quantitative anatomical phenotyping in three dimensions, and allow the calculation of locally effective midgut concentrations of applied chemicals. This atlas will provide critical insights into the evolution of the alimentary tract in lepidopterans. ‪@fraunhofer‬ ‪@universitaetgiessen‬ ‪@UniGiessenFBMedizin‬ #PreclinicalImaging #MicroCT #Imaging #Manduca #Hornworm #ManducaSexta #Entomology #WeKnowHow #TobaccoHornworm #imaging #insects #anatomy #science #lifescience #3danimation #3dart https://www.antonwindfelder.com