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Learn how to transform RGB fMRI DICOM images into grayscale using Python's `pydicom` library. This guide explains the process step-by-step and provides code examples to ensure success. --- This video is based on the question https://stackoverflow.com/q/70427525/ asked by the user 'Ben77' ( https://stackoverflow.com/u/17727087/ ) and on the answer https://stackoverflow.com/a/70427907/ provided by the user 'MrBean Bremen' ( https://stackoverflow.com/u/12480730/ ) at 'Stack Overflow' website. Thanks to these great users and Stackexchange community for their contributions. Visit these links for original content and any more details, such as alternate solutions, latest updates/developments on topic, comments, revision history etc. For example, the original title of the Question was: Trying to input a RGB fMRI DICOM image, modify it, and save it as a grayscale dicom image in Python using pydicom Also, Content (except music) licensed under CC BY-SA https://meta.stackexchange.com/help/l... The original Question post is licensed under the 'CC BY-SA 4.0' ( https://creativecommons.org/licenses/... ) license, and the original Answer post is licensed under the 'CC BY-SA 4.0' ( https://creativecommons.org/licenses/... ) license. If anything seems off to you, please feel free to write me at vlogize [AT] gmail [DOT] com. --- Converting RGB fMRI DICOM Images to Grayscale in Python with pydicom Are you looking to convert an RGB fMRI scan into a grayscale DICOM image using Python? This task can seem daunting, especially if you're running into errors while trying to modify DICOM data elements. In this post, we'll take a closer look at how to tackle this challenge using the pydicom library and ensure a smooth process for converting your images. Understanding the Problem You may find yourself needing to process medical images for research or clinical purposes. If your scan comes in RGB format and you need to save it as a grayscale image, you might encounter issues when attempting to modify pixel data. Common errors include the inability to open the modified DICOM file, indicating that it may not be a valid DICOM image. Common Issues Array Type Mismatch: The pixel array might be in the wrong format (e.g., float instead of byte). Incorrect Metadata: Setting DICOM metadata (like PhotometricInterpretation) incorrectly can cause problems. Pixel Configuration: Adjusting properties like SamplesPerPixel and BitsAllocated must align with the grayscale format. Step-by-Step Solution Step 1: Import Libraries Begin by importing the necessary libraries needed to read and manipulate DICOM files: [[See Video to Reveal this Text or Code Snippet]] Step 2: Define the RGB to Grayscale Function We will convert RGB images to grayscale using a weighted sum approach. Here’s how you can define the function: [[See Video to Reveal this Text or Code Snippet]] Step 3: Read the DICOM File Next, read the input DICOM image: [[See Video to Reveal this Text or Code Snippet]] Step 4: Convert to Grayscale Using the defined function, convert the pixel array to grayscale and ensure it's in the correct format: [[See Video to Reveal this Text or Code Snippet]] Step 5: Modify DICOM Metadata Before saving, you'll need to make sure the metadata is correctly set for a grayscale image: [[See Video to Reveal this Text or Code Snippet]] Step 6: Save the Grayscale DICOM File Finally, set the pixel data and save the new grayscale image: [[See Video to Reveal this Text or Code Snippet]] Additional Considerations Transfer Syntax: Ensure that your images are uncompressed for the code to work seamlessly. Data Attributes: Other attributes, such as ImageType, might also need adjustments depending on how you will use the modified image. Unique Identifiers: Always generate a new SOPInstanceUID to avoid conflicts with existing images. Conclusion Converting RGB fMRI DICOM images to grayscale can be achieved by following the steps outlined above. Ensure you are managing the data types correctly and set the appropriate DICOM metadata attributes to avoid common pitfalls. With this guide, you should be well-equipped to tackle similar image processing tasks using pydicom in Python. Now go ahead and give it a try on your RGB DICOM scans!