How to Isolate RNA: Total RNA Extraction Protocol for qPCR скачать в хорошем качестве

How to Isolate RNA: Total RNA Extraction Protocol for qPCR

Learn how to perform a *total RNA extraction protocol* using magnetic bead–based technology for fast, reliable RNA isolation. In this video, we demonstrate how the MagMAX mirVana Total RNA Isolation Kit enables quantitative recovery of RNA from tissue and plasma samples, supporting both manual and automated workflows. You’ll learn: The principles of bead-based isolation compared to filter-based RNA extraction. Step-by-step manual RNA extraction from tissue stored in RNAlater. Preparing reagents, lysis buffer, and magnetic bead binding steps. How to use a KingFisher Flex instrument for automated RNA purification. Applications of purified RNA in qPCR and other downstream analyses. The MagMAX mirVana kit provides consistent yields, scalability, and flexibility, making it ideal for high-throughput RNA purification in molecular biology and clinical research labs. 🔗 Learn more: https://www.thermofisher.com/us/en/ho... 📌 Chapters: 00:00 – Introduction to Total RNA Extraction 00:40 – Manual RNA Extraction Workflow 02:00 – Reagent Preparation and Bead Binding 03:15 – Washing and Elution Steps 04:30 – Automated RNA Isolation with KingFisher Flex 06:00 – RNA Purification for qPCR and Storage Related Videos: Purify Circulating Cell-Free DNA →    • How to Isolate Cell-Free DNA from Plasma |...   Qubit 4: RNA Quantitation →    • Qubit 4 Fluorometer: Accurate RNA Quantita...   Principles of PCR →    • PCR Denaturation, Annealing & Extension Ex...