[TALK 5] Fluorescence Spectroscopy and Microscale Thermophoresis- Stephen McLaughlin скачать в хорошем качестве

[TALK 5] Fluorescence Spectroscopy and Microscale Thermophoresis- Stephen McLaughlin

Fluorescence Spectroscopy and Microscale Thermophoresis Speaker: Stephen McLaughlin, MRC Laboratory of Molecular Biology, UK The LMB Biophysics Facility houses a wide range of state-of-the-art and in-house built instruments that enable the molecular characterisation of macromolecular size, shape, mass, stability, binding affinity, dynamics and interaction kinetics. The facility is primarily intended for hands-on research by students, postdocs, and group leaders, who prepare samples and make measurements. Biophysics supports users in advice on experimental techniques and results, training and in collaborative research. This video gives an overview of the fluorescence spectroscopy from the fundamentals to applications in protein stability, binding, and kinetics including techniques such fluorescence polarisation (FP), Föster resonance energy transfer (FRET) and differential scanning fluorimetry (DSF). The last quarter of the video details the theory and application of microscale thermophoresis (MST) to studies on interactions. The Biophysics Facility is headed by Chris Johnson, supported by Stephen McLaughlin. Read more about the facility here: https://www2.mrc-lmb.cam.ac.uk/resear... You can find more videos from the LMB 2022 Biophysics Techniques Lecture series here:    • Biophysical Techniques Course 2022   -- About the MRC Laboratory of Molecular Biology (LMB): The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Its scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health. More links: Official Site: https://www2.mrc-lmb.cam.ac.uk/ Facebook:   / mrc.lmb   Twitter:   / mrc_lmb   Instagram:   / mrc_lmb   LinkedIn:   / mrc-laboratory-of-molecular-biology   Click here to subscribe to MRC Laboratory of Molecular Biology on YouTube: https://www.youtube.com/user/LMBCambr...