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PEIJIAN SHI Theoretical and Empirical Conditions for Validating the Montgomery-Koyama-Smith Equation in Estimating Total Leaf Area per Shoot Leaves are the principal organs of photosynthesis, and their total area (leaf area) is a fundamental biophysical trait that directly governs a plant’s photosynthetic capacity, light interception efficiency, and energy balance. Accurate estimation of individual and total leaf area is therefore essential. The Montgomery equation establishes a proportional relationship between individual leaf area (A) and the product of leaf length and width (LW). Recently, this principle was extended to estimate the total leaf area per shoot or culm (AT). This formulation is referred to as the Montgomery-Koyama-Smith equation (MKSE). However, empirical evidence indicates that AT scales allometrically with LKSWKS, with a scaling exponent less than one. In this study, we demonstrate that the MKSE holds true under the following conditions: (i) the leaf areas of each shoot, when sorted in ascending order, follow a geometric sequence; (ii) the common ratio of these geometric sequences is consistent across shoots; (iii) the number of leaves per shoot is constant; and (iv) leaf width scales isometrically or allometrically with leaf length according to a power law. This work provides a theoretical foundation and practical guidance for the appropriate application of the MKSE in estimating total leaf area, thereby enhancing its utility in ecological and physiological studies. Bamboo Research Institute, Nanjing Forestry University, Nanjing, China