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PacBio Targeted HiFi Sequencing Approaches

Explore targeted HiFi sequencing approaches with PacBio, offering a focused and cost-effective alternative to whole genome analysis for specific genes or regions. These methods enhance scalability and affordability, whether using the benchtop Vega system or the higher-throughput Revio system, providing robust coverage of difficult-to-sequence areas. PacBio's targeted HiFi sequencing enables unambiguous, direct haplotype phasing without parental samples and delivers high-accuracy variant calling, including complex structural variants and copy number variations. This capability allows labs to consolidate multiple legacy workflows, such as Southern blotting or MLPA, into a more efficient, consistent NGS-based process, particularly valuable in clinical research and high-throughput environments. Discover three main approaches: PCR-free PureTarget for native DNA methylation and repeat expansion studies, long-read optimized HiFi target enrichment for comprehensive gene panels, and cost-effective amplicon sequencing for smaller panels or variant validation. 00:00 Introduction to Targeted HiFi Sequencing 00:20 Why Choose a Targeted Approach? 00:55 Advantages of Targeted HiFi Reads 01:36 Consolidating Legacy Molecular Workflows 02:21 Three Main Targeted HiFi Approaches 02:28 PureTarget: PCR-Free Enrichment 03:04 HiFi Target Enrichment Panels 03:39 Amplicon Sequencing for Targeted Regions 04:11 Choosing the Right Method 04:32 Benefits of Targeted HiFi Sequencing Subscribe for more insights from PacBio:    / @pacificbiosciences   Learn more about PacBio at https://pacb.com/ Legal & Trademarks: Visit https://www.pacb.com/legal-and-tradem...