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Nucleotide BLAST (BLASTn) is a powerful bioinformatics tool provided by NCBI that allows researchers to compare a DNA or RNA sequence against vast nucleotide databases such as GenBank, RefSeq, and the non-redundant (nt) database. In this video, we explain how BLASTn works conceptually and how nucleotide sequence similarity helps in identifying genes, confirming species origin, and understanding evolutionary relationships. #blast #bioinformatics #biotechnology #molecularbiology This video focuses on how a query nucleotide sequence is aligned with known sequences in databases, how alignment results are interpreted using key parameters like percent identity, query coverage, and E-value, and how these results are translated into meaningful biological conclusions. Nucleotid #moleculartechniques #ncbi #nucleotides e BLAST is widely used in molecular biology, genetics, biotechnology, and bioinformatics for applications such as gene annotation, PCR product verification, mutation detection, and species identification. This video is especially useful for BSc and MSc Biotechnology students, bioinformatics beginners, and anyone interested in learning how sequence analysis bridges experimental biology and computational research.