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In this comprehensive physiology lecture for the MBBS 2025-2026 batch at Santiniketan Medical College & Hospital, we explore the clinical and physiological complexities of blood grouping, Rh factor, and safe transfusion practices. This session is designed to help medical students bridge the gap between textbook theory and real-world clinical application. Key Topics Covered: ABO & Rh Systems: Understanding agglutinogens, agglutinins, and Landsteiner’s Laws. [00:20] The Bombay Blood Group: Chemical background of the H-antigen and its rarity in clinical practice. [01:47] Rh Factor & Pregnancy: A deep dive into Erythroblastosis Fetalis, Hydrops Fetalis, and the prevention of Rh sensitization with anti-D immunoglobulins. [11:05] Clinical Significance: Hemolytic Disease of the Newborn (HDN) and the risks of Kernicterus (bilirubin-induced neurological damage). [15:17] Blood Banking 101: Collection, storage, and the role of anti-coagulants like CPDA-1 in maintaining blood viability. [28:58] Transfusion Medicine: Indications for whole blood, packed red cells (PRBC), and fresh frozen plasma (FFP). [32:17] Hazards of Transfusion: Mismatched transfusion risks, including acute renal failure and circulatory overload (TACO/TRALI). [38:35] Paternity Disputes: Using blood groups in forensic medicine and the limitations of genetic exclusion. [26:29] SANTINIKETAN MEDICAL COLLEGE & HOSPITAL Real Classroom Demonstration/Lecture On: PHYSIOLOGY by Dr. Akash Ghosh Roy (MBBS Batch: 2025-26) Conducted on: 10-02-26