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This is a presentation by Roberta Estates entitled “Reveal Your Maternal Ancestors & Their Stories Using Mitotree and Discover – New Tools to Solve Mitochondrial DNA Puzzles”. Genealogists have wonderful new tools to use to unravel matrilineal ancestry. The mitochondrial tree of humanity has been rewritten, connecting all of us more closely than ever before on the new Mitotree and making it even easier to use mitochondrial DNA to decipher your matrilineal line. Everyone receives mitochondrial DNA only from their mother, who receives it from her mother, and so forth, all the way back in time to Mitochondrial Eve. Mitochondrial DNA is never admixed with the DNA of the other parent, so you never have to sort out which lines it comes from. We are all leaves on the twigs on the branches of the tree of humankind. The mitochondrial tree had not been updated since 2016, but now, with more than a million samples to work with, 50 times more than before, the tree structure has been expanded eight-fold. The new Mitotree and tools provide information never before available to genealogists about their matrilineal lineages. In addition to the vastly expanded genetic tree, FamilyTreeDNA has rolled out the companion MitoDiscover, a dozen fascinating chapters in your mitochondrial book. As a Million Mito Team member, Roberta will explain the challenges the team overcame to create the tree of humanity – and how it’s useful to genealogists. All genealogists can benefit from the results of the Million Mito Project, because everyone has mitochondrial DNA that holds keys to information never before available! Genealogists can also benefit from tests taken by others, like your father, to unveil your paternal grandmother’s mitochondrial DNA. Roberta steps through the process and provides ”how-to” case studies and success examples. Roberta Estes, MS, MBA, is the author of the books “DNA for Native American Genealogy” and “The Complete Guide to FamilyTreeDNA: Y-DNA, Mitochondrial, Autosomal and X-DNA” and writes the popular blog www.DNAexplain.com. She is a scientist, a National Geographic Genographic affiliate researcher, and a Million Mito team member, and is a founding pioneer in the genetic genealogy field. An avid 40-year genealogist, Roberta has written over 1700 articles at DNAexplain about genetic genealogy as well as how to combine traditional genealogy with DNA to solve those stubborn ancestor puzzles. Roberta's books are available at www.Genealogical.com and on Amazon.com.