Study of Restriction Endonucleases Enzyme-Biotechnology-Unit 2- B. Pharmacy 6 Sem-Lect.2 скачать в хорошем качестве

Study of Restriction Endonucleases Enzyme-Biotechnology-Unit 2- B. Pharmacy 6 Sem-Lect.2

For Notes Please download App: https://mbpra.courses.store/321879?ut... #Pharmaceutical_Biotechnology A restriction enzyme is a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence. Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences Products of Restriction Digestion Restriction digestion of double-stranded DNA produces two kinds of ends: Sticky ends and Blunt ends Identity of Restriction Enzymes:  EcoRI is isolated from E. coli strain RY13.  Eco refers to the genus and species (1st letter of genus; 1st two letters of specific epithet)  R is the strain of E. coli Types of Restriction Endonucleases Enzyme Type I Enzymes Type II Enzymes Type III Enzymes Applications of Restriction Endonucleases Enzyme Molecular cloning DNA mapping Gene sequencing Pulse field gel electrophoresis Restriction fragment length polymorphism (RFLP) ---------------------------------------------------------------------------------------------------------------------------------------------- My Telegram account: https://t.me/joinchat/UrMLkw_VPi5RMbO... ------------------------------------------------------------------------------------------------------------------------------------------- My Facebook Page: https://www.facebook.com/MS-Sci-Acade... ----------------------------------------------------------------------------------------------------------------------------------------------