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Confused about TBE and TAE buffers? You’re not alone! Many researchers and students often struggle to understand the differences between these two important buffers. Today, we explain why each buffer matters in gel electrophoresis and how to pick the right one for your DNA research needs. We will delve into the composition of TBE (Tris Borate EDTA) and TAE (Tris Acetate EDTA) discussing their unique properties and how they affect the separation of DNA fragments during electrophoresis. Additionally, we will provide practical tips and insights on selecting the appropriate buffer based on your specific experiment's goals. Whether you are a seasoned scientist or a beginner in the field, this video aims to clarify any confusion and enhance your understanding of these essential buffers in molecular biology. Join us for this informative discussion and elevate your knowledge of agarose gel electrophoresis! Struggling with understanding the different forms of plasmid DNA? In this video, we'll help you master the basics by walking through the characteristics of supercoiled, relaxed, linear, and multimeric DNA. From compact structures to chains of plasmids, we’ll show how each type moves through a gel and their role in biological experiments. If plasmid DNA has been confusing to you, this is the perfect place to start learning! #biotech #lablife #microbiology Related Products Tris Base - https://goldbio.com/product/3414/tris EDTA Disodium - https://goldbio.com/product/3175/edta... Agarose LE (Molecular Biology Grade) - https://www.goldbio.com/product/868/a... DNA Amplification - https://www.goldbio.com/collection/dn... Watch Next Differentiate Large And Small Strands Of DNA: Interpreting Gel Electrophoresis Results - • Differentiate Large And Small Strands Of D... How to Interpret Gel Electrophoresis Results: Different types of plasmid DNA - • How to Interpret Gel Electrophoresis Resul... Resources At Goldbio.com 10x TBE Stock Solution Protocol - https://goldbio.com/documents/3538/TB... 50x TAE Stock Solution Protocol - https://goldbio.com/documents/3490/TA... Choosing Between TAE buffer and TBE Buffer for Agarose Gel Electrophoresis - https://goldbio.com/articles/article/... Chapters: 00:00 Introduction: TBE vs. TAE in gel electrophoresis 00:18 What is agarose gel electrophoresis? 0:43 Chemical Composition: TBE vs. TAE 1:00 When to use TBE and when to use TAE 1:16 How does gel electrophoresis separate dna fragments? 1:55 What is the purpose of running buffer in gel electrophoresis? 2:54 Key Differences: Boric Acid vs. Acetic Acid 3:10 Choosing the Right Running Buffer for Your Experiment Related Products Agarose LE (Molecular Biology Grade) - https://www.goldbio.com/product/868/a... GoldBio DNA Ladders - https://www.goldbio.com/collection/dn... DNA Amplification - https://www.goldbio.com/collection/dn... Written and Presented by Karen Martin; Edited by Karen Pierce _________________________________________________________________________________________ Gold Biotechnology, Inc. - https://www.goldbio.com At GoldBio, we strive to exceed the expectations of our customers, vendors and strategic partners through quality of products, excellent service and attention to detail. Our sustainable business model and relationships with others in the supply chain allow us to provide researchers and scientists with uniquely low prices, while maintaining the highest quality in the industry. We stand behind all our products to the fullest extent with the hope that they will be used in discoveries that benefit all. Come say hi on other forms of social media! Linkedin: / gold-biotechnology-inc- Facebook: / goldbiotechnology Twitter: / goldbio Instagram: / goldbiostl