A Start to Finish Guide to Target Gene Validation Using Quantitative RT-PCR скачать в хорошем качестве

A Start to Finish Guide to Target Gene Validation Using Quantitative RT-PCR

Originally broadcast 12th September 2018 in association with Qiagen. Presented by Matthew Mule. While next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of publication. In this webinar, you will learn: • Low (1-5 genes) vs medium (~300 genes) throughput experimental design • Pros and cons of self-designed vs “off the shelf” assays • How to set up your wet lab experiments start to finish • Downloadable example step-by-step experiments with real data analysis and tutorial • Biological considerations (time series data, cell population frequency changes + more) • Examples of these techniques in publications • Common pitfalls and how to avoid them • Limitations of the technique • MIQE and publication standards Whether you are interested in a few genes or a few hundred, join Matthew Mule as he takes you through the necessary steps to validate expression levels of target genes using qRT-PCR with single gene assays and other medium-throughput platforms.