Beyond the genome: HiFi multi-omics & isoforms at PAG 33 скачать в хорошем качестве

Beyond the genome: HiFi multi-omics & isoforms at PAG 33

In this opening address from PAG 33, discover how PacBio HiFi sequencing moves beyond the genome blueprint to reveal true biology through multi-omics. PacBio Co-Founder Jonas Korlach argues that gene expression counts are insufficient metrics for understanding function and demonstrates why resolving full-length isoforms is critical. He highlights how HiFi sequencing captures the complexity of transcriptomes and mutational processes across the tree of life, contrasting these insights with the limitations of short-read RNA-seq. What you’ll learn Evaluate why gene expression counts are insufficient for understanding biological function. Examine evidence showing how short-read RNA-seq fails to capture alternative splicing complexity. Explore recent discoveries in zebrafish and the Darwin Tree of Life project enabled by HiFi data. Featured speakers Jonas Korlach, Co-Founder & Scientific Advisor (PacBio) 00:00 Opening 01:36 Welcome to PAG 33 02:32 From blueprint to true biology 04:06 Why isoforms matter 05:50 The short-read problem 06:20 Iso-Seq discoveries 08:17 Scaling to populations 09:05 Darwin Tree of Life study 10:18 Workshop preview How is isoform resolution changing your understanding of your organism? Comment below. Beyond the Bench a series celebrating the people behind scientific progress: https://www.pacb.com/blog/beyond-the-... Subscribe for more PacBio genomics content:    / @pacificbiosciences   Learn more about PacBio at https://pacb.com/ Legal & Trademarks: https://www.pacb.com/legal-and-tradem... #PAG33 #HiFiSequencing #Genomics