Illumina Single-Cell RNA Prep: Full Workflow & Data Analysis Guide скачать в хорошем качестве

Illumina Single-Cell RNA Prep: Full Workflow & Data Analysis Guide

Join the University of Chicago SCIC (scic.pme.uchicago.edu) for an in-depth technical workshop on the Illumina Single Cell 3’ RNA Prep workflow (powered by PIPseq™ technology). This session covers everything from initial experimental design to secondary data analysis using the DRAGEN pipeline. In this video, you will learn: 1. Workflow Overview: How Particle-templated Instant Partitions (PIPs) eliminate the need for microfluidics. 2. Experimental Design: How to scale from 2,000 to 100,000 cells and manage sample multiplexing. 3. Sample Prep & QC: Best practices for cell viability and nuclei suspension. 4. Data Analysis: A deep dive into the DRAGEN Single Cell RNA workflow and tertiary analysis options like Partek Flow. Speakers: Cha'Tonya Brown, PhD – Field Application Scientist (FAS), Illumina Kelly Kyle – Bioinformatics Specialist, Illumina Hosted by: The Single Cell Immunophenotyping Core (SCIC) at PME, the University of Chicago. Timestamps: 0:00 Introduction to Single-Cell Technology 10:15 The PIPseq Workflow & Equipment 25:30 Experimental Design & Scaling 40:00 Sample Preparation & QC Best Practices 55:20 Data Analysis: DRAGEN Pipeline Overview 1:10:00 Tertiary Analysis & Visualization For more information or to access these services at the SCIC, contact Fatih Abasiyanik (abasiyanik@uchicago.edu).