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USER TALK: CaTCH it if you can! CRISPRa Tracing of Therapy Resistant Cancer Cell Clones

WEBINAR: CaTCH it if you can! CRISPRa Tracing of Therapy Resistant Cancer Cell Clones ABSTRACT: The emergence of cell clones that are resistant to targeted therapies poses a significant issue in the treatment of metastatic melanoma. While founding clones are often extremely rare in a starting population, their isolation and characterization hold unique potential for understanding disease processes, uncovering novel biomarkers, and developing therapeutic concepts. In this webinar, Christian Umkehrer of Vienna’s Research Institute of Molecular Pathology describes the development and application of CaTCH (CRISPRa Tracing of Clones in Heterogeneous Cell Populations), which combines precise mapping of the lineage history of millions of cells with the ability to isolate any given clone alive from a complex population based on genetic barcodes. CaTCH enables the retrospective isolation and analysis of founding clones from heterogeneous cell populations prior to evolutionary selection. The approach uses QuantSeq 3’ mRNA-Seq to characterize the isolated clones by efficiently profiling gene expression. SPEAKER: Christian Umkehrer, PhD Scientist Research Institute of Molecular Pathology FOR MORE INFORMATION: Learn more about QuantSeq 3‘ mRNA-Seq Library Prep Kit: https://www.lexogen.com/quantseq-3mrn... Learn more about QuantSeq-Pool Sample-Barcoded 3′ mRNA-Seq Library Prep Kit for Illumina: https://www.lexogen.com/quantseq-pool... Learn more about Lexogen products: https://www.lexogen.com FOLLOW US: Facebook - / lexogen Twitter - / lexogen LinkedIn - / admin Instagram - / lexogen

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