PADIT-seq: Protein affinity to DNA by in vitro transcription and RNA sequencing скачать в хорошем качестве

PADIT-seq: Protein affinity to DNA by in vitro transcription and RNA sequencing

Revolutionizing Gene Regulation: A New Model of Transcription Factor Binding. In this exciting video, Martha Bulyk, PhD and Shubham Khetan, PhD discuss their publication in Nature, titled "Multiple overlapping binding sites determine transcription factor occupancy." This work introduces PADIT-seq, a powerful new method that maps transcription factor (TF) binding preferences across every possible 10-base-pair DNA sequence. Their findings reveal hundreds of previously undetected lower-affinity binding sites that play a crucial role in regulating gene expression. The researchers explain how traditional technologies often miss these subtle but important binding sites. They describe how overlapping DNA sequences, especially those flanking high-affinity sites, influence TF occupancy in living cells. This leads to a new model of TF binding, where occupancy is determined not by a single site but by the combined effect of multiple overlapping sites. The implications are profound, especially for understanding how noncoding genetic variants can impact gene expression and contribute to human traits and diseases. 📖 Read the full paper in Nature: https://www.nature.com/articles/s4158... Code and processed data for generating the figures are available at https://github.com/BulykLab/PADIT-seq Discover more from the Bulyk Lab: http://thebrain.bwh.harvard.edu/ 👍 Like, 💬 Comment, and 🔔 Subscribe for more cutting-edge science content! #genomics #scienceexplained #generegulation