DNA Bendability Regulates Transcription Factor Binding to Nucleosomes (PIONEAR-seq) скачать в хорошем качестве

DNA Bendability Regulates Transcription Factor Binding to Nucleosomes (PIONEAR-seq)

Unlocking the Secrets of Pioneer Transcription Factors | Featuring Luca Mariani and Martha Bulyk Recently published in Nature Structural & Molecular Biology, this research introduces PIONEAR-seq, a high-throughput biochemical assay that reveals how transcription factors interact with nucleosomes, the tightly packed structures of DNA and histones that regulate gene accessibility. Transcription factors are proteins that bind to specific DNA sequences to control gene expression. Some of these factors, known as pioneers, have the unique ability to access DNA wrapped within nucleosomes, making them essential for initiating changes in cell identity and function. However, not all DNA binding sites are equally accessible, and the rules governing this accessibility have remained unclear. Using PIONEAR-seq, the authors discovered that the broader DNA sequence context within nucleosomes plays a critical role in determining where transcription factors can bind. Surprisingly, many transcription factors show different binding behaviors on synthetic versus genomic DNA sequences, challenging long-held assumptions about pioneer factor activity.Their findings show that DNA bendability and nucleosome composition influence whether a transcription factor binds at the center or edge regions of the nucleosome. These insights challenge previous assumptions and suggest that DNA flexibility is a key regulatory feature in the genome. Watch to learn how this innovative approach is reshaping our understanding of gene regulation and chromatin dynamics. Don't forget to like, subscribe, and comment for more content! Read the full paper in Nature Structural & Molecular Biology: https://www.nature.com/articles/s4159... Code Availability: https://github.com/BulykLab/nucleosom... More exciting research from the Bulyk Lab: http://the_brain.bwh.harvard.edu/ #transcription #generegulation #technology