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OpentronsAI is an innovative software platform designed to revolutionize protocol creation and optimization for the lab automation industry. It combines a graphical user interface (GUI) with a trained large language model (LLM) and retrieval-augmented generation (RAG) technology to help users quickly generate and refine protocols. The platform features a streamlined user interface where scientists can easily select their own hardware and consumables, alongside a chat-based input system for defining experimental setups. In this webinar, we will reflect on our learnings from our previous Beta release where over 300 users developed and ran protocols over a period of three months. We will also share updated features of the newly released model including an enhanced interface that makes protocol development even more streamlined. Finally, we will give a live demo of OpentronsAI. We will walk you through the protocol development process from beginning to end, make corrections, and improve existing protocols.