DNP NMR spectroscopy for biological applications | Prof. Jiafei Mao | Session 103 скачать в хорошем качестве

DNP NMR spectroscopy for biological applications | Prof. Jiafei Mao | Session 103

During the 103rd session of the Global NMR Discussion Meetings held on June 3rd, 2025, via Zoom, Prof. Jiafei Mao from the Chinese Academy of Sciences, China, gave a talk on the topic "DNP NMR spectroscopy for biological applications". The recording serves as a tutorial. Find out more about Prof. Jiafei Mao' research : https://scholar.google.com/citations?... Abstract: Dynamic Nuclear Polarization (DNP) has revolutionized NMR spectroscopy, enabling powerful new approaches to investigate molecular structures and mechanisms in the life sciences. This tutorial offers a practical overview of biological DNP NMR spectroscopy. We begin by introducing the core components of modern DNP NMR, including instrumentation, hyperpolarization agents, sample preparation strategies, and spin dynamics, with an emphasis on their application in biological systems. Next, we highlight the established capabilities of DNP-enhanced NMR through real-world examples involving complex and challenging biological targets. We then explore emerging frontiers at the intersection of DNP NMR and life sciences, showcasing its expanding potential. Finally, we critically examine the remaining challenges and intrinsic limitations of DNP NMR spectroscopy, considering their implications for biological relevance, impact, and future significance. Content of this video : 00:00 - 05:01 Introduction on NMR in biological study context 05:02 - 08:20 DNP for improving the sensitivity of NMR 08:21 - 13:11 DNP instrumentation for solid state samples 13:12 - 15:24 Radicals for solid state DNP 15:25 - 22:06 Sample formulation for DNP 22:07 - 25:54 The impact of temperature on spectral resolution and how to improve it 25:55 - 37:13 Studies using DNP at low temperature 37:14 - 55:08 Q&A Current organizers: Adrian Draney (Creighton Uni.) Amrit Venkatesh (University of Virginia) Asif Equbal (New York Uni., Abu Dhabi) Blake Wilson (Robert Tycko Lab, NIH) Charlotte Bocquelet (HMRLab, CRMN Lyon) Diganta Sarkar (Alberta Uni.) Julie Buhl (Uni. JKU Linz) Marcel Levien (Emsley Lab, EPFL) Mengshan Ye (Miller Institute, UC Berkeley) Michael Hope (Warwick Uni.) Mouzhe Xie (Arizona State Uni.) Nikita Rao (Indian Institute of Science, Bangalore) Nesreen Elathram (Debelouchina Lab, UCSD) Tamali Nag (Uni. of Lille/Grenoble)