Plaque Reduction Neutralization Test PRNT And Microneutralization Assay скачать в хорошем качестве

Plaque Reduction Neutralization Test PRNT And Microneutralization Assay

The Plaque Reduction Neutralization Test (PRNT) and the Microneutralization Assay are laboratory techniques used to measure the neutralizing activity of antibodies against viruses. Here's a breakdown of each: Plaque Reduction Neutralization Test (PRNT): Purpose: Quantifies the titer of neutralizing antibodies in a serum sample. Method: A viral suspension is mixed with serial dilutions of the serum and incubated with a monolayer of host cells. A semi-solid overlay (like agar) is added to restrict viral spread. Readout: After incubation, plaques (areas of infected cells) are counted. The serum dilution that reduces the number of plaques by 50% (PRNT50) is used as a measure of neutralizing antibody titer. Applications: Considered the gold standard for detecting neutralizing antibodies, especially for arboviruses like dengue and Zika. Microneutralization Assay: Purpose: Measures the ability of antibodies to neutralize viruses, often used for high-throughput testing. Method: Similar to PRNT but performed in smaller wells (e.g., 96-well plates). The virus-serum mixture is incubated with host cells, and viral replication is assessed using colorimetric, fluorescent, or luminescent readouts. Advantages: Faster and more scalable than PRNT, making it suitable for large-scale studies #Neutralization