Measuring the concentration and purity of a plasmid DNA sample скачать в хорошем качестве

Measuring the concentration and purity of a plasmid DNA sample

Hello Everyone, I’ve created these videos primarily as instructional aides for new students, interns, and trainees in my research group. While many of the methods are generally applicable, I am of course producing these demonstrations with the materials, reagents, and equipment that are available in our lab. Also, I’ll occasionally reference the locations of items in our lab. In this video, I go over how to measure the concentration and purity of a plasmid DNA sample using an Eppendorf Biophotometer. Whenever I’m handling a DNA sample, I try to work under a flame with good aseptic technique. While this isn’t entirely necessary (DNA is relatively stable), I try to take reasonable steps to keep dust and other contaminants out of my DNA. Dust contains nucleases, which will slowly degrade your sample when it is thawed out. Moreover, it’s worth emphasizing that even a tiny quantity of particulate matter in your DNA sample can have a huge impact on your measurement (either your purity or your yield). I presume that this is because the amount of light scattered by insoluble material may be considerably greater than the amount of light absorbed by your DNA. About me: I am currently (at the time of uploading this video) a doctoral student in the Chemical and Biomolecular Engineering department at The Ohio State University. My advisor is Dr. David Wood, and our research group specializes in protein engineering and downstream processing for the biotechnology industry. I’m also very new at producing instructional videos so I apologize in advance if these are not of professional quality. For instance, in my first few videos I did not know how to get rid of the timestamp (which is wrong), however I posted them anyways. I’m sure these will get better with time, but for now you’ll just have to bear with me. Please keep comments civil and apolitical. You are of course welcome to ask questions, however it is unlikely that I will answer them if you are not a member of my research group. Thanks for watching, Steven