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Watch the Full Video at https://www.jove.com/v/2490/c-elegans.... C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays - a 2 minute Preview of the Experimental Protocol Amanda Kauffman, Lance Parsons, Geneva Stein, Airon Wills, Rachel Kaletsky, Coleen Murphy Princeton University, Department of Molecular Biology, Lewis-Sigler Institute for Integrative Genomics; Princeton University, Lewis-Sigler Institute for Integrative Genomics; Here we describe methods to test C. elegans associative learning and short- and long-term associative memory. These population assays employ the worms abilities to chemotax toward volatile odorants, and form positive associations upon pairing food with the chemoattractant butanone. Increasing the number of conditioning periods induces long-term memory. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments