Pharmaceutics/Lecture-11 C/Centrifugation/Microcentrifuges/High-speed centrifuges/Ultracentrifuges скачать в хорошем качестве

Pharmaceutics/Lecture-11 C/Centrifugation/Microcentrifuges/High-speed centrifuges/Ultracentrifuges

Welcome to PharmaLecture – Your Trusted Source for Quality Pharmacy Education “Centrifugation is the process of separating lighter portions of a solution, mixture or suspension from the heavier portions by centrifugal force”. The rate of centrifugation is specified by the angular velocity measured in revolutions per minute (RPM). Microcentrifuges Microcentrifuges are used to process small volumes of biological molecules, cells, or nuclei. Microcentrifuge tubes generally hold 0.5 - 2.0 mL of liquid, and are spun at maximum angular speeds of 12,000–13,000 rpm. High-speed centrifuges High-speed or superspeed centrifuges can handle larger sample volumes, from a few tens of millilitres to several litres. Additionally, larger centrifuges can also reach higher angular velocities (around 30,000 rpm). The rotors may come with different adapters to hold various sizes of test tubes, bottles, or microtiter plates. Ultracentrifuges Ultracentrifugation makes use of high centrifugal force for studying properties of biological particles. Compared to microcentrifuges or high-speed centrifuges, ultracentrifuges can isolate much smaller particles, including ribosomes, proteins, and viruses. Ultracentrifuges can reach maximum angular velocities in excess of 70,000 rpm. Applications Industrial centrifuges are used: 1.For separating solids from liquids, liquid-liquid separation, and liquid-solid separation. 2.Removing fat from milk to produce skimmed milk 3.Separation of urine components and blood components in forensic and research labs. 4.In laboratories performing biochemical analyses on body fluids, centrifuges are routinely used: To separate blood cells from serum/plasma. 5.To separate sediment from urine 6.To measure the volume fraction of erythrocytes in blood (the hematocrit) For any Question Whats app +923038489485 Email jspharma116@gmail.com Subscribe now and equip yourself with the knowledge and confidence to succeed in the dynamic field of pharmacy.