Reproducible differentiation of ovarian support cells as a novel infertility treatment | REPROCELL скачать в хорошем качестве

Reproducible differentiation of ovarian support cells as a novel infertility treatment | REPROCELL

Reproductive diseases impact at least 10% of the population, but limited research funding has reflected healthcare disparities and stalled innovation. The use of human induced pluripotent stem cells (hiPSCs) shows great promise in generating resourceful solutions for accelerating innovation in reproductive health. Recently, we demonstrated a novel approach for fast and efficient differentiation of ovarian support cells (OSCs), generating a versatile platform for basic research, as well as clinical applications. This technology was recently applied to improve in vitro maturation (IVM) of human oocytes, a method called OSC-IVM. We have now demonstrated in-depth characterization of OSCs through a comprehensive single-cell RNA-sequencing analysis of over 10 batches. Transcription factor-mediated differentiation generates cells in different stages of ovarian development and folliculogenesis.  Moreover, substituting reagents with appropriate higher-quality alternatives, in particular by incorporating laminin-521 as a differentiation matrix, leads to a scalable, reproducible, and pure population of OSCs, with the capability of inducing consistent in vitro maturation of human oocytes. Finally, by applying the modified protocol to a newly generated clinical-grade hiPSC clone, we demonstrated not only the robustness of the process but also the full translation of a research prototype into a clinical solution ready to be applied to support infertility treatment. Our findings demonstrate first-time clinical translation of an hiPSC-derived therapy to advance women’s health, paving the way for its clinical application. https://www.reprocell.com/ ____________________________________________________________________________ Follow us on LinkedIn:   / reprocell   Follow us on Twitter:   / reprocellglobal   ____________________________________________________________________________ Browse our product catalog: https://www.reprocell.com/product-cat... Got a research project you are looking to outsource? Contact our scientists to arrange a free consultation: https://www.reprocell.com/contact