Chromatography column volumes (CVs) & how to calculate them скачать в хорошем качестве

Chromatography column volumes (CVs) & how to calculate them

Protein chromatographers talk about CVs a lot – column volumes, that is! We talk in terms of CVs and not just total volumes, because this lets us apply protocols from one column to a column that’s bigger or smaller. The easiest way to find the column volume is to look it up in the product manual, etc. But, you can also calculate it using information that’s often provided in the column name: Chromatography column names often contain dimensions in the format: Internal Diameter (i.d.)/Bed Height (BH). i.d. is usually given in mm, but, annoyingly, the BH may be given in mm (for small columns) or cm (for big ones) To find the Column Volume (CV): 1. Convert to cm as needed 2. Convert i.d. to radius (r) 3. Calculate the CV: πr^2 CV = π x (0.5 x i.d.)^2 × ВН For example, my favorite column, Superdex 200 10/300 GL: 3.14 × (0.5 cm)^2 × 30 cm = 23.55 cm^3 = 23.55 mL And our HiLoad Superdex 75 16/600: 3.14 x (1.6 cm)^2 x 60 cm = 120.6 cm^3 = 120.6 mL But then, our HiPrep 26/10 desalting column has its BH in cm… 3.14 x (1.3 cm)^2 x 10 cm = 53 cm^3 = 53 mL PS – special thanks to Caroline Duffy from Cytiva for answering a bunch of my chromatography questions! More on chromatography: http://bit.ly/proteinpurificationtech More about all sorts of techniques: https://thebumblingbiochemist.com/let...    more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com                                   #scicomm #biochemistry #molecularbiology #biology #sciencelife #science #realtimechem