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CONTACT's PRODUCE SAFETY WEBINAR SERIES, SEASON 4, WEBINAR 26 Title: Research to Reality ~ Biological Soil Amendments Description: Season 4 of CONTACT's Produce Safety Webinar Series features Drs. Keith Scheider, Laurel Dunn, and Manan Sharma present on biological soil amendments of animal origin. This webinar provides a sneak peek of CONTACT Objective 2 data and findings. Biological soil amendments of animal origin (BSAAO), like livestock manure, compost, and heat-treated amendments, are frequently used as organic fertilizers to improve soil's physical and chemical properties and nutrient content. BSAAOs are also a known reservoir for enteric bacterial pathogens like enterohemorrhagic E. coli and Salmonella. Numerous mechanisms (splash, direct contact with soil, and dust) transfer pathogens to fruits and vegetables, and foodborne outbreaks have been linked to manure-associated contamination of leafy greens with E. coli O157:H7. Soils amended with BSAAOs supply nutrients that enteric pathogens may utilize directly or indirectly to survive for extended durations. Past studies illustrate that amended soils extend the survival of non-pathogenic E. coli compared to unamended soils. It has also been shown that regionality (climate, soil type, method of BSAAO application practices) dramatically influences the survival of pathogens in the soil. Funding: This work is supported by the Specialty Crops Research Initiative 2020-51181-32157 from the USDA National Institute of Food and Agriculture. Any opinions, findings, conclusions, or recommendations expressed in this recommendation are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.