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Sara McArdle presents different ways of changing ROIs, and demonstrates how to create regions around pancreatic islets. Files https://drive.google.com/drive/folder... and a related presentation https://docs.google.com/presentation/... are available for download. 00:00:00 Sara McArdle introduces ROI manipulation tools 00:00:25 Example: ROIs around tumors see • QuPath Workshop From Samples to Knowledge ... 00:00:42 Example: ROIs around vessels 00:01:00 Human pancreas anatomy 00:01:48 Changing maximum display range 00:01:58 Finding tissue outline with a thresholder 00:03:13 Loading classifier and creating objects 00:03:36 Minimum object size 00:03:51 Creating pixel classifier for pancreatic islets 00:04:52 Tips for making a good classifier 00:06:41 Selecting objects by classification 00:07:21 Pixel classifier settings 00:07:40 Explanation of Scales 00:09:02 Dealing with artefacts 00:09:56 Question: why use the polyline tool? 00:11:20 The special case of Ignore* classes 00:12:09 Creating objects from pixel classifier 00:12:39 Duplicating image for a fresh start 00:13:21 Don't split your tissue but do split islets 00:14:12 Shift + F to fill annotations 00:15:08 Making peri-islet regions 00:16:52 Effect of removing interior 00:17:21 Effect of constraining to parent 00:18:23 Making an exocrine region 00:18:48 Making inverse annotation 00:19:14 Resolving overlapping regions 00:20:10 Merging islets 00:21:50 Use scripting to do more complex operations 00:22:46 Manual corrections 00:23:13 Tip for cell detections 00:23:30 Making use of object hierarchy Please follow this document to get everything you need to fully participate in this training course (software, files, cheat sheets, and additional resources): https://docs.google.com/document/d/1V... If you have any questions about the videos or the training course, please ask on this image.sc forum post. https://forum.image.sc/t/qupath-train... The more information you can include about your question, the more helpful our answers can be.